Which animation studio, at its peak, had 250 employees and was located in Manhattan?

Fleischer Studios is known for its significant contributions to the animation industry during the early 20th century, particularly in the 1920s and 1930s. At its peak, the studio employed around 250 people and was located in Manhattan, which was at the time a major hub for animation production. The studio gained fame for creating iconic characters like Betty Boop and Popeye, as well as pioneering techniques such as rotoscoping, where live-action footage is traced to create animation. This innovative approach and the studio's distinctive art style helped differentiate it from contemporaries, making its employee count and Manhattan location noteworthy aspects of its history.

In contrast, the other studios listed have different historical contexts and locations; for instance, Disney Studios, while also highly influential, is primarily associated with California. Warner Bros. Animation, although a key player in animation history, does not line up with the specifics of the question regarding peak employment and location. Pencil Test Studios, being a more contemporary studio, does not match the timeframe or prominence of Fleischer Studios during its peak.